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Advantages and disadvantages of ultrasonic cell disruptor

In the development process of the ultrasonic cell disruptor, it has the characteristics of high-activity pulverization of cells, which improves the efficiency of sample processing and the rate of bacterial cell disruption. Once the sample is processed, the rate of pulverization of bacterial cells can reach more than 99%. And the physiological activity of the target protein is protected to the greatest extent, and some preliminary results have been achieved.

Ultrasonic cell disruptor uses ultra-high pressure energy to disrupt cells. Compared with ultrasound, the advantages of the ultrasonic cell crusher are: maintain cell viability, save time, reduce sample loss, and can process a large number of samples. Therefore, the continuous development and improvement of ultrasonic cell pulverizers have made considerable contributions to large-scale production.

In the biotechnology industry, the cost of separation and purification often accounts for 60% to 90% of the cost of the entire process. my country's biotechnology industry is dominated by the traditional fermentation industry. Product separation and purification is the most important stage of the fermentation industry. It is the downstream of the entire fermentation production line. It not only determines the yield, but also determines the quality and safety of the product. This part includes many operations: separation of cells and fermentation broth, broken cells to release intracellular products, membrane separation to remove cell debris, etc.

Ultrasonic disruption by ultrasonic cell disruptor is currently the most commonly used disruption method in biological production. Fermentation broth of different strains. The effect of ultrasonic disintegration is quite different. Generally, bacilli are easier to break than cocci, and gram-negative bacterial cells are easier to break than gram-positive bacterial cells, and the effect on yeast is less. Advantages: simple operation, less liquid loss, suitable for laboratory scale. Disadvantages: high cost; easy to cause a sharp rise in temperature; in large-scale operation, sound energy transmission and heat dissipation are difficult, and the generated chemical radical groups are easy to inactivate the product.

There are many manufacturers of ultrasonic cell disruptors, and their products are different in technology content, which directly leads to the difference in the lifespan of ultrasonic cell disrupters.

How long can the ultrasonic cell disrupter be used? This is actually a question that is not easy to say. How long the ultrasonic cell disrupter can be used is related to many factors, not only the quality of the ultrasonic cell disrupter, but also the use of the ultrasonic cell disruptor Operations and so on are related. Generally speaking, the better the quality of the ultrasonic cell disrupter, the longer its corresponding use time, on the contrary, it is shorter; (for example, the ultrasonic cell disrupter produced by Ningbo Uviz has a general service life of 3 to 5 years. Look like).

The daily use of the ultrasonic cell disrupter will also affect its lifespan. Generally, it will be used for a longer time if it is properly maintained and operated correctly. On the contrary, it will reduce the service life of the ultrasonic cell disrupter. So we are When using the ultrasonic cell disrupter, you must read its manual before using it, so that the ultrasonic cell disrupter we bought can last longer.

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